Type 2 rhinovirus infection of cultured human tracheal epithelial cells: role of LDL
receptor.
Suzuki, Tomoko, Mutsuo Yamaya, Masahito Kamanaka, Yu X. Jia, Katsutoshi
Nakayama, Masayoshi Hosoda, Norihiro Yamada, Hidekazu Nishimura, Kiyohisa
Sekizawa, and Hidetada Sasaki.
1Department of Geriatric and Respiratory Medicine, Tohoku University School of
Medicine, Sendai 980«hyphen»8574; 3Virus Center, Clinical Research Division, Sendai
National Hospital, Sendai 983«hyphen»0045; and 2Department of Pulmonary Medicine,
Institute of Clinical Medicine, University of Tsukuba, Tsukuba 305«hyphen»8575,
Japan
APStracts 7:0267L, 2000.
To examine the role of the low-density lipoprotein (LDL) receptor on minor group
human rhinovirus (RV) infection, primary cultures of human tracheal epithelial cells were
infected with a minor group (RV2) or a major group (RV14) RV. Viral infection was
confirmed by showing with PCR that viral titers in supernatants and lysates from infected
cells increased with time. RV2 and RV14 increased expression of mRNA and protein of
the LDL receptor on the cells and the cytokine production. RV2 induced activation of
transcription factors SP1 and nuclear factor-?B (NF-?B). An antibody to the LDL
receptor inhibited RV2 infection and RV2-induced cytokine production without an effect
on RV14 infection and RV14-induced cytokine production. These findings imply that
RV2 upregulates LDL receptor expression on airway epithelial cells, thereby increasing
susceptibility to minor group RV infection. LDL receptor expression and cytokine
production may be mediated, in part, via activation of transcription factors by RV2.
These events may be important in airway inflammation after minor group RV infection in
asthma.
Received 4 July 2000; accepted in final form 16 October 2000
APS Manuscript Number L221-0.
Article publication pending Am J Physiol Lung Cell Mol Physiol
ISSN 1080-4757 Copyright 2000 The American Physiological Society.
Published in APStracts on 30 November 2000