High-fat hypocaloric diet modifies carbohydrate utilization of obese rats during
weight loss.
Cha, Ming C., Julia A. Johnson, Chang-Yun Hsu, and Carol N. Boozer.
1New York Obesity Research Center, St. Luke's-Roosevelt Hospital, New York
10025; and 2Institute of Human Nutrition, Department of Medicine, College of
Physicians and Surgeons, Columbia University, New York, New York 10032
APStracts 8:0020E, 2001.
The effects of fat content in the hypocaloric diet on whole body glucose oxidation and
adipocyte glucose transport were investigated in two animal-feeding experiments. Diet-
induced obese rats were food restricted to 75% of their previous energy intakes with
either a high (45% by calorie) or a low (12% by calorie) corn oil diet for 9 wk
(experiment 1) or 10 days (experiment 2). The losses of body weight (P < 0.05) and
adipose depot weight (P < 0.05) were less in the 45% compared with the 12% fat group.
During the dynamic phase of weight loss (day 10 of food restriction), plasma glucose and
insulin concentrations were higher (P < 0.05) in the 45% than those in the 12% fat
group. Whole body carbohydrate oxidation rate in response to an oral load of glucose was
increased (P < 0.001) by food restriction in both dietary groups. However, carbohydrate
oxidation rates were lower (P < 0.01) in the 45% than in the 12% fat-fed rats during the
weight loss period. Adipocyte glucose transport was greater (P < 0.02) in the 45% than
in the 12% fat group in an intra-abdominal adipose depot but not in subcutaneous fat.
These data suggest that dietary fat content modifies whole body glucose oxidation and
intra-abdominal adipocyte glucose uptake during weight loss.
Received 22 August 2000; accepted in final form 22 January 2001
APS Manuscript Number E397-0.
Article publication pending Am J Physiol Endocrinol Metab
ISSN 1080-4757 Copyright 2001 The American Physiological Society.
Published in APStracts on 27 February 2001