Genetic selection of mice for high voluntary wheel-running: effect on skeletal
muscle glucose uptake.
Dumke, C. L., J. S. Rhodes, T. Garland Jr, E. Maslowski, J. G. Swallow, A. C. Gazdag H,
and G. D. Cartee.
1Biodynamics Laboratory and Department of Kinesiology, 2Department of Zoology,
and 3Department of Nutritional Sciences, University of Wisconsin, Madison, Wisconsin
53706
APStracts 8:0272A, 2001.
Effects of genetic selection for high wheel-running activity (17th generation) and access
to running wheels on skeletal muscle glucose uptake were studied in mice with the
following treatments for 8 wk: 1) access to unlocked wheels; 2) same as 1, but wheels
locked 48 h before glucose uptake measurement; or 3) wheels always locked. Selected
mice ran more than random-bred (nonselected) mice (8-wk mean ± SE = 8,243 ± 711 vs.
3,719 ± 233 revolutions/day). Body weight was 5-13% lower for selected vs. nonselected
groups. Fat pad/body wt was ~40% lower for selected vs. nonselected and for unlocked
vs. locked groups. Insulin-stimulated glucose uptake and fat pad/body wt were inversely
correlated for isolated soleus (r = «minus»0.333; P < 0.005), but not extensor digitorum
longus (EDL) or epitrochlearis muscles. Insulin-stimulated glucose uptake was higher in
EDL (P < 0.02) for selected vs. nonselected mice. Glucose uptake did not differ by
wheel-group, and amount of running did not correlate with glucose uptake for any
muscle. Wheel running by mice did not enhance subsequent glucose uptake by isolated
muscles.
Received 31 January 2001; accepted in final form 1 May 2001
APS Manuscript Number A0101-1.
Article publication pending J Appl Physiol
ISSN 1080-4757 Copyright 2001 The American Physiological Society.
Published in APStracts on 18 June 2001