Effect of mechanical deformation of neutrophils on their CD18/ICAM-1-dependent adhesion.
Anderson, Gregory J., William T. Roswit, Michael J. Holtzman, James C. Hogg, and Stephan F. Van Eeden.
1Pulmonary Research Laboratory, University of British Columbia, St Paul's Hospital, Vancouver, British Columbia, Canada V6Z 1Y6; and 2Pulmonary Division, Washington University Medical School, St. Louis, Missouri 63110
APStracts 8:0296A, 2001.
Mechanical deformation of polymorphonuclear leukocytes (PMN) changes their expression of the surface adhesion molecule CD11b/CD18. We tested the hypothesis that mechanical deformation of PMN enhances their adhesiveness. Purified human PMN were deformed through either 5- or 3-µm polycarbonate membrane filters and allowed to adhere to 96-well plates coated with human recombinant intercellular adhesion molecule-1 (ICAM-1). Flow cytometric studies showed that deformation of PMN increased CD11b/CD18 expression (P < 0.01). PMN adhesion to ICAM-1-coated plates was dependent on the magnitude of cell deformation (5 µm, 63.8 ± 8.1%, P < 0.04; 3 µm, 232.4 ± 20.9%, P < 0.01). Priming of PMN (0.5 nM N-formyl-methionyl-leucyl-phenylalanine) before deformation (5 µm) increased PMN adhesion (63.8 ± 8.1 vs. 105.3 ± 16.4%; P < 0.04). Stimulation (5% zymosan-activated plasma) of PMN after deformation resulted in increased adhesion, and the degree of increase was dependent on the magnitude of PMN deformation (stimulation, 50.6 ± 4%; 5-µm filtration and stimulation, 62.9 ± 6.6%; 3-µm filtration and stimulation, 249.9 ± 24.2%; P < 0.01). This study shows that mechanical deformation of PMN causes an increase in PMN adhesiveness to ICAM-1 that was enhanced by both priming of PMN before deformation and stimulation after cell deformation.
Received 2 November 2000; accepted in final form 25 April 2001
APS Manuscript Number A1035-0.
Article publication pending J Appl Physiol
ISSN 1080-4757 Copyright 2001 The American Physiological Society.
Published in APStracts on 18 June 2001