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Department Research

Molecular Neurobiology
Core Facility

Neurobiology and Anatomy > Research > Molecular Neurobiology Core Facility

Room MSB 7.402
Coordinator: M. Neal Waxham, Ph.D. | E-Mail
Telephone: 713.500.5621

Molecular NeurobiologyThe molecular neurobiology core facility was established to support work in the areas of protein chemistry, neurochemistry and molecular biology. Several pieces of equipment form the core of this facility. A Beckman binary HPLC system is available, driven by the System Gold software package, with detection capabilities that include: Shimadzu fluorometric and/or Coulochem electrochemical analysis of neurotransmitters and related substances, two Beckman UV detectors, one standard variable wavelength and the second a photodiode array detector capable of generating spectrum scans "on-the-fly. There is also a FPLC instrument with accompanying UV detector for the purification of proteins. The core is further equipped with a Beckman XPN ultracentrifuge with numerous swinging bucket and fixed angle rotors, a Beckman Avanti JE centrifuge, a Beckman TL1 table top ultracentrifuge and a recently upgraded Beckman LS-6000 scintillation counter. The molecular neurobiology core's most recent acquisition is a GE Healthcare Trio+ flat-bed imaging system run by ImageQuant software made possible by an equipment grant from the NIH/NCRR.  This imaging system is for quantitative analysis of fluorescent or radiolabeled proteins or nucleic acids analyzed on one- and two-dimensional gels.  As part of this system, the DeCyder software package for quantifying data using the 2D-difference in gel electrophoresis (2D-DIGE) applications was established.


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