The molecular neurobiology core facility was established to support work in the areas of protein chemistry, neurochemistry and molecular biology. Several pieces of equipment form the core of this facility. A Beckman binary HPLC system is available, driven by the System Gold software package, with detection capabilities that include: Shimadzu fluorometric and/or Coulochem electrochemical analysis of neurotransmitters and related substances, two Beckman UV detectors, one standard variable wavelength and the second a photodiode array detector capable of generating spectrum scans "on-the-fly. There is also a FPLC instrument with accompanying UV detector for the purification of proteins. The core is further equipped with a Beckman XPN ultracentrifuge with numerous swinging bucket and fixed angle rotors, a Beckman Avanti JE centrifuge, a Beckman TL1 table top ultracentrifuge and a recently upgraded Beckman LS-6000 scintillation counter. The molecular neurobiology core's most recent acquisition is a GE Healthcare Trio+ flat-bed imaging system run by ImageQuant software made possible by an equipment grant from the NIH/NCRR. This imaging system is for quantitative analysis of fluorescent or radiolabeled proteins or nucleic acids analyzed on one- and two-dimensional gels. As part of this system, the DeCyder software package for quantifying data using the 2D-difference in gel electrophoresis (2D-DIGE) applications was established.