Office of Technology Management
Improved Method
of Immunoglobulin Adsorption to Selected Surfaces
Summary:
This invention is directed to the selection and fractionation of IgG species,
and to the processes for achieving preferential adsorption of the F(c)
portion of IgG molecules to selected surfaces relative to their F(ab) portions.
This invention enables the fabrication of antibody-tagged surfaces with the adsorbed antibodies arranged in a conformation which allows for proper interaction with antigens. The F(c) portion of an IgG species will selectively bind with a positively charged adsorption surface if the F(c) portion has an acidic pH. The F(c) portion of an IgG species will selectively bind with a negatively charged adsorption surface if the F(c) portion has a basic pI. Thus the preferred conformational binding is ensured by selection of the correct IgG and surface combination. This method could improve the specificity of clinical diagnostic tools such as adsorption immunochromatography and immunodetection.
This invention enables the fabrication of antibody-tagged surfaces with the adsorbed antibodies arranged in a conformation which allows for proper interaction with antigens. The F(c) portion of an IgG species will selectively bind with a positively charged adsorption surface if the F(c) portion has an acidic pH. The F(c) portion of an IgG species will selectively bind with a negatively charged adsorption surface if the F(c) portion has a basic pI. Thus the preferred conformational binding is ensured by selection of the correct IgG and surface combination. This method could improve the specificity of clinical diagnostic tools such as adsorption immunochromatography and immunodetection.
Technology Status:
This
patent is available for licensing from UT-Houston Health Science Center.
Patent Status:
Patent
No. 4,880,751
Inventor:
William
Geoghegan, Ph.D.