The Department of Pathology & Laboratory Medicine

Publications

Sudhir Paul, Ph.D.

A. Publications:

Paul, S., and Planque, S.(2006) Antibody engineering. In: Nature Encyclopedia of Life Sciences. John Wiley & Sons, Ltd: Chichester http://www.els.net/ [DOI: 10.1038/npg.els.0001278].
Paul, S., Nishiyama, Y., Planque, S., and Taguchi, H. Theory of proteolytic antibody occurrence. Immunol. Lett. 103:8-16, 2006. Pubmed
Hanson, C.V., Nishiyama, Y., and Paul, S. Catalytic antibodies and their applications. Curr Opin Biotechnol. 16:631-636, 2005.
Nishiyama, Y., Mitsuda, Y., Taguchi, H., Planque, S., Hara, M., Karle, S., Hanson, C.V., Taizo, U., and Paul, S. Broadly distributed reactivity of proteins coordinated with specific ligand binding activity. J. Mol. Recognit. 18:295-306, 2005. Pubmed
Paul, S., Nishiyama, Y., Planque, S., Karle, S., Taguchi, H., Hanson, C., and Weksler. M.E. Antibodies as Defensive Enzymes. Springer Semin. Immun. 26:485–503, 2005. Pubmed
Paul, S., Karle, S., Planque, S., Taguchi, H., Salas, M., Nishiyama, Y., Handy, B., Hunter, R., Edmundson, A., and Hanson, C. Naturally occurring proteolytic antibodies: Selective IgM-catalyzed hydrolysis of HIV gp120. J. Biol. Chem. 279:39611-39619, 2004. Pubmed
Taguchi, H., Keck, Z., Foung, S.K.H., Paul, S. and Nishiyama, Y. Antibody light chain-catalyzed hydrolysis of a hepatitis C virus peptide. Bioorg. Med. Chem. Lett. 14:4529-4532, 2004. Pubmed
Voice, J., Donnelly, S., Dorsam, G., Dolganov, G., Paul, S. and Goetzl, E.J. c-Maf and JunB mediation of Th2 differentiation induced by the type 2 G protein-coupled receptor (VPAC2) for vasoactive intestinal peptide. J. Immunol. 172:7289-7296, 2004. Pubmed
Keck, Z., Sung, V.M.H., Perkins, S., Rowe, J., Paul, S., Liang, T.J., Lai, M.M.C. and Foung, S.K.H. Human monoclonal antibody to hepatitis C virus E1 glycoprotein that blocks virus attachment and viral infectivity. J. Virol. 78: 7257–7263, 2004. Pubmed
Le, A., Dasgupta, S., Planque, S., Paul, S. and Thiagarajan, P. Lupus-derived antiprothrombin autoantibodies from a V gene phage display library are specific for the kringle 2 domain of prothrombin. Biochemistry. 43:4047-4054, 2004. Pubmed
Planque, S., Bangale, Y., Karle, S., Taguchi, H., Poindexter, B., Bick, R., Edmundson, A., Nishiyama, Y. and Paul, S. Ontogeny of proteolytic immunity: IgM serine proteases. J. Biol. Chem.279:14024-14032, 2004. Pubmed
Nishiyama, Y., Bhatia, G., Bangale, Y., Planque, S., Mitsuda, Y., Taguchi, H., Karle, S., and Paul, S. Towards selective covalent inactivation of pathogenic antibodies: A phosphonate diester analog of vasoactive intestinal peptide that Inactivates catalytic autoantibodies. J. Biol. Chem. 279:7877–7883, 2004. Pubmed
Karle, S., Planque, S., Nishiyama, Y., Taguchi, H., Zhou, Y.-X., Salas, M., Lake, D., Thiagarajan, P., Arnett, F., Hanson, C.V., and Paul, S. Cross-clade HIV-1 neutralization by an antibody fragment from a lupus phage display library. AIDS. 18:329–347, 2004.
Rangan, S.K., Liu, R., Brune, D., Planque, S., Paul, S., and Sierks, M.R. Degradation of β-amyloid by Proteolytic Antibody Light Chains. Biochemistry. 42:14328-14334, 2003. Pubmed
Paul, S., Planque, S., Zhou, Y.-X., Taguchi, H., Bhatia, G., Karle, S., Hanson, C., and Nishiyama, Y. Specific HIV gp120 cleaving antibodies induced by covalently reactive analog of gp120. J. Biol. Chem. 278:20429-20435, 2003. PubMed
Planque, S., Taguchi, H., Burr, G., Bhatia, G., Karle, S., Zhou, Y.-X., Nishiyama, Y., and Paul, S. Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen bonding activity. J. Biol. Chem. 278:20436 - 20443, 2003. PubMed
Bangale, Y., Karle, S., Zhou, Y.-X., Li, L., Kalaga, R., and Paul, S. VIPase autoantibodies in Fas-defective mice and patients with autoimmune disease. FASEB J. 17:628-635, 2003. PubMed
Karle, S., Nishiyama, Y., Zhou, Y.-X., Luo, J., Planque, S., Hanson, C., and Paul, S. Carrier-dependent specificity of antibodies to a conserved peptide determinant of gp120. Vaccine. 21:1213–1218, 2003. PubMed
Planque, S., Zhou, Y.-X., Nishiyama, Y., Sinha, M., O'Connor-McCourt, M., Arnett, F. C., and Paul, S. Autoantibodies to the epidermal growth factor receptor in systemic sclerosis, lupus and autoimmune mice. FASEB J. 17:136-143 2003. PubMed
Voice, J. K., Grinninger, C., Kong, Y., Bangale, Y., Paul, S., and Goetzl, E. J. Roles of vasoactive intestinal peptide (VIP) in the expression of different immune phenotypes by wild-type mice and T cell-targeted type II VIP receptor transgenic mice. J. Immunol. 170:308-314, 2003. PubMed
Bangale, Y., Cavill, D., Gordon, T., Planque, S., Taguchi, H., Bhatia, G., Nishiyama, Y., Arnett, F., and Paul, S. Vasoactive intestinal peptide binding autoantibodies in autoimmune humans and mice. Peptides. 23:2251-2257, 2002. PubMed
Taguchi, H., Burr, G., Karle, S., Planque, S., Zhou, Y.-X., Paul, S., and Nishiyama, Y. A mechanism-based probe for gp120-hydrolyzing antibodies. Bioorg. Med. Chem. Lett. 12:3167–3170, 2002. PubMed
Zhou, Y.-X., Karle, S., Taguchi, H., Planque, S., Nishiyama, Y., and Paul, S. Prospects for immunotherapeutic proteolytic antibodies. J. Immunol. Methods. 269:257-268, 2002. PubMed
Nishiyama, Y., Taguchi, H., Luo, J., Zhou, Y.-Z., Burr, G., Karle, S., and Paul, S. Covalent reactivity of a phosphonate monophenyl ester with serine proteinases: An overlooked feature of oxyanionic transition state analogs. Arch. Biochim. Biophys. 402:281–288, 2002. PubMed
Aleksandrova, E. S., Koralevski, F., Titov, M. I., Demin, A.V., Kozyr', A. V., Kolesnikov, A. V., Tramontano, A., Paul, S., Thomas, D., Gabibov, A. G., Gnuchev, N. V., and Friboulet, A. A structure-activity study of a catalytic antiidiotypic antibody to the human erythrocyte acetylcholinesterase. Bioorg Khim. 28:118-125, 2002. PubMed
Berisha, H. I., Bratut, M., Bangale, Y., Colasurdo, G., Paul, S., and Said, S. I. New evidence for transmitter role of VIP in the airways: Impaired airway relaxation by a catalytic antibody to VIP. Pulm. Pharmacol. Ther. 15:121-127, 2002. PubMed
Alexandrova, E. S., Koralewski, F., Titov, M. I., Demin, A. V., Ignatova, A. N., Kozyr, A. V., Kolesnikov, A. V., Tramontano, A., Paul, S., Thomas, D., Gabibov, A. G., and Friboulet, A. Catalysis of esterolytic reactions by the anti-idiotypic antibody against human erythrocyte acetylcholinesterase. Dokl. Biochem. Biophys. 377:75-78, 2001. PubMed
Paul, S., Tramontano, A., Gololobov, G., Zhou, Y.-X., Taguchi, H., Karle, S., Nishiyama, Y., Planque, S., and George, S. Phosphonate ester probes for proteolytic antibodies. J. Biol. Chem. 276:28314-28320, 2001. PubMed
Kolesnikov, A. V., Kozyr, A. V., Alexandrova, E. S., Koralewski, F., Demin, A. V., Titov, M. I., Avalle, B., Tramontano, A., Paul, S., Thomas, D., Gabibov, A. G., and Friboulet, A. Enzyme mimicry by the anti-idiotypic antibody approach. Proc. Natl. Acad. Sci. 97:13526-13531, 2000. PubMed
Thiagarajan, P., Dannenbring, R., Matsuura, K., Tramontano, A., Gololobov, G., and Paul, S. A monoclonal antibody light chain with prothrombinase activity. Biochemistry. 39:6459-6465, 2000. PubMed
Thiagarajan, P. and Paul, S. Prothrombin cleaving antibody light chains. In: Chemical Immunology: Catalytic Antibodies. (S. Karger AG, Basel, Switzerland) Ed., S. Paul. Vol. 77, pp. 115-129, 2000. Karger
Tramontano, A., Gololobov, G., and Paul, S. Proteolytic antibodies: Origins, selection and induction. In: Chemical Immunology: Catalytic Antibodies. (S. Karger AG, Basel, Switzerland) Ed., S. Paul. Vol. 77, pp. 1-17, 2000. Karger
Paul, S., Ed. Chemical Immunology: Catalytic Antibodies. (S. Karger AG, Basel, Switzerland) Vol. 77, pp. 1-158, 2000. Karger
Paul, S., Tramontano, A., Sarma, U., Thomas, D., and Gabibov, A., (Eds.) Proceedings of the 3rd International Conference on Catalytic and Super Antibodies. Appl. Biochem. Biotechnol. 83:1-318, 2000.
Li, L., Kalaga, R., and Paul, S. Proteolytic components of serum IgG preparations. Clin. Exp. Immunol. 120:261-266, 2000. PubMed
Gololobov, G., Tramontano, A., and Paul, S. Nucleophilic proteolytic antibodies. Appl. Biochem. Biotech. 83:221-232, 2000. PubMed
Tramontano, A., Ivanov, B., Gololobov, G., and Paul, S. Inhibition and labeling of enzymes and abzymes by phosphonate esters. Appl. Biochem. Biotech. 83:233-243, 2000. PubMed
Paul, S., Kalaga, R., Gololobov, G., and Brenneman, D. Natural catalytic immunity is not restricted to autoantigenic substrates: Identification of a human immunodeficiency virus gp120 cleaving antibody light chain. Appl. Biochem. Biotech. 83:71-84, 2000. PubMed
Paul, S. and Tramontano, A. Antibody Catalysis - A Super Activity. The Immunologist. 7:163-168, 1999.
Gololobov, G., Sun, M., and Paul, S. Innate antibody catalysis. Mol. Immunol. 36:1215-1222, 1999. PubMed
Paul, S., Ed. Autoimmune Reactions (Humana Press, Totowa, NJ) pp 1 - 438,1998.
Bosilevac, J. M., Gilchrist, C.A., Jankowski, P.E., Paul, S., Rees, A. R., and Hinrichs, S. H. Inhibition of ATF1 and CREB activated transcription by an intracellular single chain Fv fragment. J. Biol. Chem. 273:16874-16879, 1998. PubMed
Ikezaki, H., Paul, S., Alkan-Onyuksel, H., Patel, M., Gao, X.-P., and Rubinstein, I. Vasodilation elicited by liposomal VIP is unimpeded by anti-VIP antibody in hamster cheek pouch. Am. J. Physiol. 275:R56-R62, 1998. PubMed
Paul, S. Autoantibody catalysis: No longer hostage to Occam's Razor. Ann. New York Acad. Sci. 865:246-254, 1998. PubMed
Gololobov, G., Noda, Y., Sherman, S., Rubinstein, I., Baranowska-Kortylewicz, J., and Paul, S. Stabilization of vasoactive intestinal peptide by lipids. J. Pharmacol. Exp. Ther. 285:753 - 758, 1998. PubMed
Kohler, H. and Paul, S. Superantibodies: New players in innate and adaptive immunity. Immunol. Today. 19: 221 - 227, 1998. PubMed
Paul, S. VIPASE. In: Handbook of proteolytic enzymes, Barrett A, Woessner F, Rawlings N., Eds. (Academic Press, London, England) pp. 533 - 535, 1998.
Paul, S., Friboulet, A., Gabibov, S., and Thomas, D., Eds. Whither Catalytic Antibodies? Proceeding of 2nd International symposium on Catalytic Antibodies and Antibody Engineering. Appl. Biochem. Biotechnol. 75: 1 - 150, 1998.
Paul, S. Protein engineering. In: Molecular Biotechniques. Walker J, Ed. (Humana Press, Totowa, NJ), Chapter 43, pp 547 - 566, 1998.
Paul, S. Relevance of catalytic anti-VIP antibodies to the airway. In: Pro-inflammatory and anti-inflammatory peptides, Lung Biology in Health and Disease Series, Sami Said, Ed. (Marcel Dekker, New York). Chapter 19, pp 459-475, 1997.
Sun, M., Gao, Q.-S., Kirnarskiy, L., Rees, A., and Paul, S. Cleavage specificity of a proteolytic antibody light chain and effects of the heavy chain variable domain. J. Mol. Biol. 271:374-385, 1997. PubMed
Kohler, H., Paul, S., and Marchalonis, J. Multifunctional variable domains. The Immunologist. 5:98-103, 1997.
Paul, S., Li, L., Kalaga, R., O'Dell, R.E., Dannenbring, Jr, R.E., Swindells, S., Hinrichs, S., Caturegli, P., and Rose, N. Characterization of thyroglobulin-directed and polyreactive catalytic antibodies in autoimmune disease. J. Immunol. 159:1530-1536, 1997. PubMed
Sun, M. and Paul, S. Altered cleavage site preference of a proteolytic antibody light chain induced by denaturation. FEBS Lett. 407:289-290, 1997. PubMed
Tyutyulkova, S., Gao, Q.-S., Thompson, A., Rennard, S., and Paul, S. Efficient vasoactive intestinal polypeptide hydrolyzing autoantibody light chains selected by phage display. Biochimica. Biophysica. Acta. 1316:217-223, 1996. PubMed
Paul, S. Natural catalytic antibodies. Mol. Biotechnol. 5:197-207, 1996. PubMed
Paul, S. Proteolytic antibodies. Isr. J. Chem. 36:207-214, 1996.
Li, L., Sun, M., Gao, Q.-S., and Paul, S. Low level formation of potent catalytic IgG fragments mediated by disulfide bond instability. Mol. Immunol. 33:593-600, 1996. PubMed
Suzuki, H., Noda, Y., Gao, X., Séjourné, F., Alkan-Önyuksel, H., Paul, S., and Rubinstein, I. Encapsulation of VIP into liposomes restores vasorelaxant in hypertension in situ. Am. J. Physiol. (Heart Circ Physiol 40) 271:H282-H287, 1996.
Li, L., Paul, S., Tyutyulkova, S., Kazatchkine, M., and Kaveri, S. Catalytic activity of anti-thyroglobulin antibodies. J. Immunol. 154:3328-3332, 1995. PubMed
Paul, S., Li, L., Kalaga, R., Wilkins-Stevens, P., Stevens, F. J., and Solomon, A. Natural catalytic antibodies: Peptide hydrolyzing activities of Bence Jones proteins and VL fragment. J. Biol. Chem. 270:15257-15261, 1995. PubMed
Tyutyulkova, S., Gao, Q.-S., and Paul, S. Selection of human immunoglobulin light chains from a phage display library. Antibody Engineering Protocols. Ed., Paul S. (Methods in Molecular Biology Series, Humana Press, Totowa, NJ) 51:377-394, 1995.
Gao, Q.-S. and Paul, S. Molecular cloning of anti-ground state proteolytic antibody fragments. Antibody Engineering Protocols. Ed., Paul S. (Methods in Molecular Biology Series, Humana Press, Totowa, NJ). 51:281-296, 1995.
Paul, S., ed. Antibody Engineering Protocols. (Methods in Molecular Biology Series, Humana Press, Totowa, NJ).51:1-450, 1995.
Suzuki, H., Noda, Y., Paul, S., Gao, X.-P., and Rubinstein, I. Encapsulation of vasoactive intestinal peptide into liposomes: Effects on vasodilation in vivo. Life Sciences. 57:1451-1457, 1995. PubMed
Kalaga, R., Li, L., O'Dell, J., and Paul, S. Unexpected presence of polyreactive catalytic antibodies in IgG from unimmunized donors and decreased levels in rheumatoid arthritis. J. Immunol. 155:2695-2702, 1995. PubMed
Gao, Q.-S., Sun, M., Rees, A., and Paul, S. Site-directed mutagenesis of proteolytic antibody light chain. J. Mol. Biol. 253:658-664, 1995. PubMed

B. Patents

Autoantibodies which enhance the rate of a chemical reaction. (1993) US patent 5,236,836; Sudhir Paul
Inhibitors of catalytic antibodies. (1993) US patent 5,194,585; Sudhir Paul, Michel J. Powell, Richard J. Massey
Catalytic antibody components. (1993) US patent 5,229,272; Sudhir Paul, Michael J. Powell, Richard J. Massey, John H. Kenten
Monoclonal antibody and antibody components elicited to a polypeptide antigen ground state. (1994) US patent 5,318,897; Sudhir Paul
Compositions and methods for catalyzing hydrolysis of HIV gp120. (2000) US patent 6,156,541; Sudhir Paul, Ravishankar Kalaga
Assay methods and kits for diagnosing autoimmune disease. (2000) US patent 6,130,049; Sudhir Paul, Ravishankar Kalaga
Catalytic antibody components. (1997) US patent 5,658,753; Sudhir Paul, Michael J. Powell, Richard J. Massey, John H. Kenten
Autoantibodies which enhance the rate of a chemical reaction. (1997) US patent 5,599,538; Sudhir Paul, Lan Li, Srini Kaveri
Autoantibodies which enhance the rate of a chemical reaction. (1997) US patent 5,602,015; Sudhir Paul
Method of delivering a vasoactive intestinal polypeptide, an encapsulated vasoactive intestinal polypeptide, and a method of making the encapsulated vasoactive intestinal polypeptide. (1998) US patent 5,770,570; Sudhir Paul, Yasuko Noda, Israel Rubinstein
Methods for identifying inducers and inhibitors of proteolytic antibodies, compositions and their uses. (2001) US patent 6,235,714; Sudhir Paul, Gennady Gololobov, Larry J. Smith
Inhibitors of catalytic antibodies. (2000) US patent 6,048,717; Sudhir Paul, Michel J. Powell, Richard J. Massey
Methods for identifying inducers and inhibitors of proteolytic antibodies, compositions and their uses. (2005) US patent 6,855,528; Sudhir Paul, Gennady Gololobov, Larry J. Smith
Covalently reactive transition state analogs and methods of use thereof. (2005) U.S. Patent 6,855,804; Sudhir Paul and Yasuhiro Nishiyama
Covalent attachment of ligands to nucleophilic proteins guided by non-covalent binding. U.S. provisional patent application, March 2003; U.S. patent application 60/457,293, March 26, 2004; Sudhir Paul
Lupus antibodies for passive immunotherapy of HIV/AIDS. U.S. provisional patent application, March 2003; U.S.patent application 60/457,570, March 2004; Sudhir Paul
Proteolytic and Covalent Antibodies. U.S. provisional patent application, March 2004; U.S. patent application 60/458,063, May 2004; Sudhir Paul and Yasuhiro Nishiyama
Methods for identifying inducers and inhibitors of proteolytic antibodies, compositions and their uses. (2005) US patent 6,855,528 B2; Sudhir Paul, Gennady Gololobov, Larry J. Smith
Covalently reactive transition state analogs and methods of use thereof. (2005) U.S. Patent 6,855,804 CIP Patent Application No. 10/114,716; Sudhir Paul and Yasuhiro Nishiyama.
Catalytic Immunoglobulins. (2005) U.S. provisional patent application filed July 13, 2005; Sudhir Paul, Yasuhiro Nishiyama, Carl V. Hanson, Marc Weksler

Yasuhiro Nishiyama, Ph.D.

A. Publications:

Kurita K, Hirakawa M, Aida K, Yang J, Nishiyama Y. Trimethylsilylated chitosan: A convenient precursor for chemical modifications. Chem Lett 2003 Nov;32(11):1074–1075.
Nishiyama, Y., Murakami, T., Shikama, S., Kurita, K., Yamamoto, N.: Anti-HIV-1 peptides derived from partial amino acid sequences of CC-chemokine RANTES. Bioorg Med Chem 2002 Dec;10(12): 4113–4117, 2002.
Kurita K, Mori S, Nishiyama Y, Harata M. N-Alkylation of chitin and some characteristics of the novel derivatives. Polym Bull 2002 Apr;48(2):159–166.
Kurita, K., Hayakawa, M., Nishiyama, Y., Harata, M.: Polymeric asymmetric reducing agents: preparation and reducing performance of chitosan/dihydronicotinamide conjugates having L- and D-phenylalanine spacer arms. Carbohydrate Polym 2002 Jan;47(1): 7–14.
Nishiyama, Y., Ishizuka, S., Kurita, K.: O-(7-Azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate–1-hydroxy-7-azabenzotriazole–copper (II) chloride: a promising epimerization-free segment coupling system for peptide synthesis. Tetrahedron Lett 2001 Dec 10;42(50): 8789–8791.
Nishiyama, Y., Ishizuka, K., Shikama, S., and Kurita, K.: An efficient synthesis of N-tert-butoxycarbonyl-O-cyclohexyl-L-tyrosine. Chem. Pharm. Bull. 49: 233–235, 2001.
Nishiyama, Y., Shikama, S., Morita, K. and Kurita, K.: Cyclohexyl ether as a new hydroxy-protecting group for serine and threonine in peptide synthesis. J. Chem. Soc., Perkin Trans. 1. 1949–1954, 2000.
Kurita, K., Kojima, T., Nishiyama, Y., and Shimojoh, M.: Synthesis and some properties of nonnatural amino polysaccharides: Branched chitin and chitosan. Macromolecules 33: 4711-4716, 2000.
Kurita, K., Uno, M., Saito, Y., and Nishiyama, Y.: Regioselectivity in protection of chitosan with the phthaloyl group. Chitin Chitosan Res. 6: 43-50, 2000.
Nishiyama, Y., Yoshikawa, T., Ohara, N. Kurita, K., Hojo, K., Kamada, H., Tsutsumi, Y., Mayumi, T., and Kawasaki, K.: A conjugate from a laminin-related peptide, Tyr-Ile-Gly-Ser-Arg, and chitosan: Efficient and regioselective conjugation and significant inhibitory activity against experimental cancer metastasis. J. Chem. Soc., Perkin Trans. 1. 1161-1165, 2000.
Hojo, K., Maeda, M., Mu, Y., Kamada, H., Tsutsumi, Y., Nishiyama, Y., Yoshikawa, T., Kurita, K., Block, L. H., Mayumi, T., and Kawasaki, K.: Facile synthesis of a chitosan hybrid of a laminin-related peptide and its antimetastatic effect in mice. J. Pharm. Pharmacol. 52: 67-73, 2000.
Joshi, S., Tsuda, Y., Shintomi, N., Kondo, H., Nishiyama, Y., Iwama, M., Ohgi, K., Irie, M., and Okada, Y.: Amino acids and peptides. LVII. Synthetic peptide with a sequence of ribonuclease from Sulfolobus solfataricus, SSR (1–62), does not function as an RNase. FEBS Lett. 468: 11-14, 2000.
Nishiyama, Y., Ishizuka, S., Mori, T., and Kurita, K.: O-(N-Succinimidyl)-1,1,3,3-tetramethyluronium tetrafluoroborate–N-hydroxysuccinimide–CuCl2: A facile and reliable system for racemization-free coupling of peptides having a carboxy-terminal N-methylamino acid. Chem. Pharm. Bull. 48: 442-444, 2000.
Kurita, K., Kaji, Y., Mori, T., and Nishiyama, Y.: Enzymatic degradation of b-chitin: Susceptibility and the influence of deacetylation. Carbohydrate Polym. 42: 19-21, 2000.
Kurita, K., Hirakawa, M., and Nishiyama, Y.: Silylated chitin: A new organosoluble precursor for facile modifications and film casting. Chem. Lett. 771-772, 1999.
Kurita, K., Amemiya, J., Mori, T., and Nishiyama, Y.: Comb-shaped chitosan derivatives having oligo(ethylene glycol) side chains. Polym. Bull. 42: 387-393, 1999.
Hojo, K., Maeda, M., Mu, Y., Kamada, H., Tsutsumi, Y., Nishiyama, Y., Yoshikawa, T., Kurita, K., Block, L. H., Mayumi, T., and Kawasaki, K.: Preparation of a chitosan hybrid of an antimetastatic laminin-related peptide. Pharm. Pharmacol. Commun. 5: 277-280, 1999.
Nishiyama, Y., Murakami, T., Kurita, K., and Yamamoto, N.: Low-molecular-weight anti-HIV-1 peptides from the amino-terminal sequence of RANTES: Possible lead compounds for coreceptor-directed anti-HIV-1 agents. Bioorg. Med. Chem. Lett. 9: 1357-1360,1999.
Nishiyama, Y., Tanaka, M., Saito, S., Ishizuka, S., Mori, T., and Kurita, K.: A racemization-free coupling method for peptides having N-methylamino acids at the carboxy-termini. Chem. Pharm. Bull. 47: 576-578, 1999.
Nishiyama, Y., Yoshikawa, T., Kurita, K., Hojo, K., Kamada, H., Tsutsumi, Y., Mayumi, T., and Kawasaki, K.: Regioselective conjugation of chitosan with a laminin-related peptide, Tyr-Ile-Gly-Ser-Arg, and evaluation of its inhibitory effect on experimental cancer metastasis. Chem. Pharm. Bull. 47: 451-453, 1999.
Nishiyama, Y. and Kurita, K.: Cyclohexyl ether as a new hydroxy-protecting group for serine in solid-phase peptide synthesis. Tetrahedron Lett. 40: 927-930, 1999.
Kurita, K., Shimada, K., Nishiyama, Y., Shimojoh, M., and Nishimura, S.: Nonnatural branched polysaccharides: Synthesis and properties of chitin and chitosan having a-mannoside branches. Macromolecules 31: 4764-4769, 1998.
Narahara, M., Nishida, K., Ohtsubo, S., Okamoto, M., Nakagawa, S., Suga, S., Ebisutani, C., Nishiyama, Y., Okada, Y., Itoh, N., Mutou, N., Tanaka, K., Hama, T. and Miyake, M.: Growth inhibitory factor/metallothionein-III in the brain of macular mutant mice. Res. Commun. Biochem. Cell Mol. Biol. 2: 55-68, 1998.
Nishiyama, Y., Yoshida, Y., Mori, T., Ishii, S., and Kurita, K.: Asymmetric reduction with chitosan/dihydronicotinamide conjugates: Influence of L-alanine spacer arms on reducing performance. React. Funct. Polym. 37: 83-91, 1998.
Kurita, K., Kojima, T., Munakata, T., Akao, H., Mori, T., Nishiyama, Y., and Shimojoh, M.: Preparation of nonnatural branched chitin and chitosan. Chem. Lett. 317-318, 1998.
Nishiyama, Y., Murakami, T., Kurita, K., and Yamamoto, N.: Synthesis of some peptides corresponding to the active region of RANTES for chemotaxis and evaluation of their anti-human immunodeficiency virus-1 activity. Chem. Pharm. Bull. 45: 2125-2127, 1997.
Kurita, K., Akao, H., Kobayashi, M., Mori, T., and Nishiyama, Y.: Regioselective introduction of b-galactoside branches into chitosan and chitin. Polym. Bull. 39: 543-549, 1997.
Kurita, K., Yoshino, H., Nishimura, S., Ishii, S., Mori, T., and Nishiyama Y.: Mercapto-chitins: A new type of supports for effective immobilization of acid phosphatase. Carbohydrate Polym. 32: 171-175, 1997.
Yokoi, T., Taguchi, H., Nishiyama, Y., Igarashi, K, Kasuya, F. and Okada Y.: Amino acids and peptides. Part 48. Studies on the structure of an unexpected reaction product from dipeptidyl chloromethyl ketone during acid hydrolysis. J. Chem. Res. (S)10-11, (M),1997.
Nishiyama, Y., Shintomi, N., Kondo, Y., Izumi, T. and Okada Y.: Amino acids and peptides. Part 42. Application of the 2-adamantyloxycarbonyl (2-Adoc) group to the protection of the imidazole function of histidine in peptide synthesis. J. Chem. Soc., Perkin Trans. 1. 2309-2313, 1995.
Nishiyama, Y., Shintomi, N., Kondo, Y. and Okada Y.: Application of the 2-adamantyloxycarbonyl (2-Adoc) group to the protection of the imidazole function of histidine in peptide synthesis. J. Chem. Soc., Chem. Commun. 2515-2516, 1994.
Nishiyama, Y., Shintomi, N., Kondo, Y., and Okada, Y.: Amino acids and peptides. Part 38. Development of a new amino-protecting group, 2-adamantyloxycarbonyl, and its application to peptide synthesis. J. Chem. Soc., Perkin Trans. 1. 3201-3207, 1994.
Nishiyama, Y. and Okada, Y.: Temporary Na-deprotection/reprotection procedure to facilitate the purification of protected peptide fragments for use in convergent solid phase peptide synthesis. Tetrahedron Lett. 35: 7409-7412, 1994.
Okada, Y., Taguchi, H., Nishiyama, Y., and Yokoi T.: Simple approach towards the synthesis of 5-methyl-2-hydroxypyrazine derivatives from dipeptidyl chloromethyl ketones. Tetrahedron Lett. 35: 1231-1234, 1994.
Taguchi, H., Yokoi, T., Kasuya, F., Nishiyama, Y., Fukui, M., and Okada, Y.: Unexpected reaction of dipeptidyl chloromethyl ketone during acid hydrolysis. J. Chem. Soc., Chem. Commun. 247, 1994.
Taguchi, H., Nishiyama, Y., Camargo, A. C. M., and Okada Y.: Amino acids and peptides. XXXVI. Synthesis of enkephalin chloromethyl ketone and evaluation of its inhibitory activity against endopeptidase 22.19. Chem. Pharm. Bull. 41: 2038-2039, 1993.
Nishiyama, Y. and Okada Y.: Development of a new amino-protecting group, 2-adamantyloxycarbonyl (2-Adoc), and its application to solid-phase synthesis of protected peptides. J. Chem. Soc., Chem. Commun. 1083-1084, 1993.
Matsumoto, S., Nishiyama, Y., Okada, Y., Min, K.-S, Onosaka, S. and Tanaka K.: Amino acids and peptides. XXXV. Synthesis of mouse metallothionein I. (2). Synthesis of a nonacosapeptide corresponding to N-terminal sequence 1-29 (a-fragment) of mouse metallothionein I and related peptides and examination of their heavy metal-binding properties. Chem. Pharm. Bull. 40: 2701-2706, 1992.
Matsumoto, S., Nakayama, S., Nishiyama, Y., Okada, Y., Min, K.-S. Onosaka, S., and Tanaka, K.: Amino acids and peptides. XXXIV. Synthesis of mouse metallothionein I. (1). Synthesis of dotriacontapeptide corresponding to C-terminal sequence 30-61 (b-fragment) of mouse metallothionein I and related peptides and examination of their heavy metal-binding properties. Chem. Pharm. Bull. 40: 2694-2700, 1992.
Nishiyama, Y., Nakayama, S. Okada, Y., Min, K.-S., Onosaka, S. and Tanaka, K.: Amino acids and peptides. XXVI. Synthesis of Agaricus bisporus metallothionein and related peptides and examination of their heavy metal-binding properties. Chem. Pharm. Bull. 38: 2112-2117, 1990.
Okada, Y., Matsuno, Y., Nishiyama, Y., Tsuda, Y., Iguchi, S., Min, K.-S., Onosaka, S., and Tanaka, K.: Amino acids and peptides. XXIV. Synthesis of Neurospora crassa metallothionein and related cysteine-containing peptides and examination of their heavy metal-binding properties. Chem. Pharm. Bull. 37: 2322-2329, 1989.
Nishiyama, Y.: 2-Adamantyl chloroformate. In Electronic Encyclopedia of Reagents in Organic Synthesis. New reagents of release 3–4, 2003. Eds. Paquette, L.; Crich, D.; Fuchs, P. L.; Wipf, P.; John Wiley and Sons, England, 2003.
Taguchi, H. and Nishiyama, Y.: 3-Bromocyclohexene. In Electronic Encyclopedia of Reagents in Organic Synthesis. New reagents of release 3–4, 2003. Eds. Paquette, L.; Crich, D.; Fuchs, P. L.; Wipf, P.; John Wiley and Sons, England, 2003.
Nishiyama, Y.: O-(N-Succinimidyl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TSTU). In Electronic Encyclopedia of Reagents in Organic Synthesis. New reagents of release 1, 2002. Eds. Paquette, L.; Rigby, J.; Roush, W.; Wipf, P.; John Wiley and Sons, England, 2002.
Okada, Y.; Nishiyama, Y. Chapter 1. Neurohypophyseal hormones and their precursors. In Protein Chemistry–Molecular Structures and Functions– Vol. 11. Hormonal Proteins and Polyamines, Kitagawa, K.; Igarashi, K. Eds. Hirokawa Publishing, Tokyo, 1996, p.1–19 (in Japanese).

Keri C. Smith, Ph.D.

A. Publications:

Csencsits, K., Lu, G., and Bishop, D. K. Alloreactive CD8+ T cells are resistant to anti-CD40L therapy in T-bet-/- and IL-12p35/p40-/-, but not IL-12Rbeta2-/- mice (In preparation).
Csencsits, K., Wood, S. C., Lu, G, Stahl, G. L., and Bishop, D. K. Role of complement in allograft rejection and inductive monoclonal antibody therapy. (In preparation).
Csencsits, K., Wood, S. C., Lu, G., Faust, S. M., Brigstock, D., Eichwald, E. J., Orosz, C. G., and Bishop, D. K. 2005. Transforming growth factor-beta induced connective tissue growth factor and chronic allograft rejection. (Am. J. Transplant. In press.)
Csencsits, K., Wood, S. C., Lu, G., and Bishop, D. K. 2005. TGF-beta gene is associated with the development of regulatory cells. Am. J. Transplant. 5: 2378-2384.
Csencsits, K., Wood, S. C., Lu, G., Magee, J. C., Eichwald, E. J., Chang, C.-H., and Bishop, D. K. 2005. Graft rejection mediated by CD4+ T cells via indirect recognition of alloantigen is associated with dominant Th2 response. Eur. J. Immunol. 35: 843-851.
Nathan, M. J., Mold, J. E., Wood, S. C., Csencsits, K. Lu, G, Eichwald, E. J., and Bishop, D. K. 2004. Requirement for donor and recipient CD40 expression in cardiac allograft rejection: induction of Th1 responses and influence of donor-derived dendritic cells. J Immunol. 172: 6626-6633.
Csencsits, K. L., and Pascual, D. W. 2002. Absence of L-selectin delays mucosal B cell responses in non-intestinal effector tissues. J. Immunol. 169: 5645-5659.
Csencsits, K. L., Jutila, M. A., and Pascual, D. W. 2002. Mucosal addressin expression and naive lymphocyte binding interactions vary among the cranial, oral, and nasal-associated lymphoid tissues. Eur. J. Immunol. 32: 3029-3039.
Csencsits, K. L., Walters, N., and Pascual, D. W., 2001. Cutting Edge: Dichotomy of homing receptor dependence by mucosal effector B cells:  vs. L-selectin. J. Immunol. 167: 2441-2445.
Wu, Y., Wang, X., Csencsits, K. L., Haddad, A., Walters, N., and Pascual, D. W. 2001. M-cell targeted DNA vaccination. Proc. Natl. Acad. Sci., USA 98: 9318-9323.
Wu,Y., Boysun, M., Csencsits, K.L., and Pascual, D.W. 2000. Gene transfer facilitated by a cellular targeting molecule, reovirus protein 1. Gene Therapy 7: 61-69.
Csencsits, K. L., Jutila, M. A., and Pascual, D. W. 1999. Nasal-associated lymphoid tissue (NALT): phenotypic and functional evidence for the primary role of peripheral node addressin in naive lymphocyte adhesion to HEV in a mucosal site. J. Immunol. 163: 1382-1389.
Csencsits, K. L. and Bishop, D. K. 2002. Contrasting alloreactive CD4 and CD8 T cells: there’s more to it than MHC restriction. Am. J. Transplant 3: 107-115.
Csencsits, K. and Bishop, D. K. 2003. Overview of cytokine structure and function. In: Immunobiology of Organ Transplantation. Editors: D. S. Wilkes and W. J. Burlingame. Kluwer Academic Publishers, New York. p. 405-435.

Hiroaki Taguchi, Ph.D.

A. Publications:

Okada, Y.; Tsukatani, M.; Taguchi, H.; Yokoi, T.; Bryant, S. D. and Lazarus, L. H. Amino Acids and Peptides. LII. Design and Synthesis of Opioid Mimetics Containing a Pyrazinone Ring and Examination of Their Opioid Receptor Binding Activity. Chem. Pharm. Bull., 1998, 46, 1374-1382.
Yokoi, T.; Taguchi, H.; Nishiyama, Y.; Igarashi, K.; Kasuya, F and Okada, Y. Amino acids and peptides. Part 48. Studies on the structure of an unexpected reaction product from dipeptidyl chloromethyl ketone during acid hydrolysis. J. Chem. Res. 1997, (S)10–11.
Okada, Y.; Taguchi, H. and Yokoi, T. Amino Acids and Peptides. XLVII. Facile Synthesis of Flavacol, Deoxymuta-aspergillic Acid and Optically Active Deoxyaspergillic Acid from Dipeptidyl Aldehydes. Chem. Pharm. Bull., 1996, 44, 2259-2262.
Taguchi, H.; Yokoi, T. and Okada, Y. Amino Acids and Peptides. XLVI. Introduction of Carboxyl Function into Pyrazinone by using Newly Developed Procedure for Pyrazinone Ring Formation: Observation of Immediate Decarboxylation. Chem. Pharm. Bull., 1996, 44, 2037-2041.
Okada, Y.; Taguchi, H. and Yokoi, T. Total Synthesis of Optically Active Deoxyaspergillic Acid from Dipeptidyl Aldehyde. Tetrahedron Lett., 1996, 37, 2249-2252.
Nakamura, M.; Tomoo, Koji.; Ishida, T.; Taguchi, H.; Tsuda, Y.; Okada, Y.; Okunomiya, A.; Wanaka, K. and Okamoto, S. Design of noncovalent trypsin inhibitor based on the X-ray crystal structure of the complex. Biochem. Biophys. Res. Commun., 1995, 213, 583-587.
Taguchi, H.; Yokoi, T.; Tsukatani, M. and Okada, Y. Amino Acids and Peptides. XLI. Facile Synthesis of 5-Methyl-2(1H)-pyrazinone Derivatives from Dipeptidyl Chloromethyl Ketones. Tetrahedron, 1995, 51, 7361-7372.
Taguchi, H.; Hirano, K.; Yokoi, T.; Asada, K. and Okada, Y. : Amino Acids and Peptides. XL. Synthesis of Ac-Tyr-Val-Ala-Asp-MCA using Newly Developed Acetylating Reagent. Chem. Pharm. Bull., 1995, 43, 1336-1339.
Okada, Y.; Taguchi, H.; Nishiyama, Y. and Yokoi T. Simple approach towards the synthesis of 5-methyl-2-hydroxypyrazine derivatives from dipeptidyl chloromethyl ketones. Tetrahedron Lett. 1994, 35, 1231–1234.
Taguchi, H.; Yokoi, T.; Kasuya, F.; Nishiyama, Y.; Fukui, M. and Okada, Y. Unexpected reaction of dipeptidyl chloromethyl ketone during acid hydrolysis. J. Chem. Soc., Chem. Commun. 1994, 247.
Taguchi, H.; Nishiyama, Y.; Camargo, A. C. M. and Okada Y. Amino acids and peptides. XXXVI. Synthesis of enkephalin chloromethyl ketone and evaluation of its inhibitory activity against endopeptidase 22.19. Chem. Pharm. Bull. 1993, 41, 2038–2039.
Teno, N.; Wanaka, K.; Okada, Y.; Taguchi, H.; Okamoto, U.; Hijikata-Okunomiya, A. and Okamoto, S. Development of Active Center-Directed Plasmin and Plasma Kallikrein Inhibitors and Studied on the Structure Activity Relationship. Chem. Pharm. Bull., 1993, 41, 1079-1090.
Taguchi, H. and Nishiyama, Y. 3-Bromocyclohexene. In Electronic Encyclopedia of Reagents in Organic Synthesis, Paquette, L.; Rigby, J.; Roush, W.; Wipf, P. Eds., John Wiley and Sons, England, in press.

B. Patents:

Preparation of calcium salts of 1,5-benzodiazepine derivatives as gastric acid secretion inhibitors and gastrin and CCK-B antagonists. Application date 12/1/2000. Application#: WO 2000-JP8511. Publication date 6/7/2001. Publication#: WO 0140197. Inventors: Shinozaki, Katsuo; Murata, Masakazu; Maeda, Kiyoto; Taguchi, Hiroaki; Kawase, Nobuo; Miura, Naoyoshi. Applicant: Zeria Pharmaceutical Co., Ltd.
Production of pyrazine derivatives. Application date: 6/11/1993. Application#: JP19930166161. Publication date: 12/20/1994. Publication#: JP6345741. Inventors: Hiroaki Taguchi, Yasuhiro Nishiyama, Toshio Yokoi, Fumiyo Kasuya, Miyoshi Fukui, Yoshio Okada. Applicant: KANEBO Co., Ltd.