Protein mass spectrometry-based analysis of complex protein and peptide mixtures using a modified MALDI - TOF mass spectrometer (SELDI technology - Ciphergen). Services include protein identification using protease finger technology and protein profiling usin SELDI technology.
High-throughput real-time quantitative PCR analysis of gene or transcript levels in DNA or RNA samples. New assay design of real-time qPCR primers and probes. Automated quantitative ELISA determinations from protein samples using the Meso Scale technology has recently been added.
Knock-out Mouse Core Facility - Services include transfection and selection of ES cells, microinjection of targeted ES clones, and generation of chimeras.
Provide the genetics expertise to support a wide variety of investigators with variable degrees of genetic knowledge. Services includes harvesting and banking of patients’ DNA samples. Provide the infrastructure to conduct genetic research, access to equipment, and the expertise needed to carry out high throughput genotyping and sequencing. Provide support with the experiment design and management of large volume databases and the statistical support needed to perform complex genetic analyses.
Flourescence microscopy and imaging - confocal scanning laser microscope for flourescence, reflectance, and transmittance; real time quantitative flourescence microscopy; and crystal biotech video edge detector to measure muscle cell contractility.
Automated DNA sequencing, oligonucleotide synthesis, and PCR technologies; and BIAcore system for surface plasmon resonance studies of protein-DNA and protein-protein interactions.
