Protein quantification for complex samples is available using Meso Scale Discovery’s electrochemiluminescence reader.
Meso Scale- Assays provided by Meso Scale have 4-logs of linearity with detection limits in the low to sub-pico gram/ml range, depending on the assay. This assay can be multiplexed to as many as 10 assays/well on a 96-well Meso Scale plate. The ability to multiplex depends upon the expected antigen concentration range for each assay and potential cross-reactivity among Abs & Ags. The strengths are multiplexing of up to 42 samples in duplicate/plate, high sensitivity and a broad 4-log linear quantification range. The only drawbacks are the 6 hour total time to process a plate for the ELISA assays (mostly incubations and wash steps).
Data Analysis- Data will be provided in an Excel workbook with the appropriate figures and post-run analyzed data from the Meso Scale software. It is up to the investigator to complete data analysis in line with experiment requirements.