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QGCL References & Links

References

Nucleic acids

  1. Bustin SA (2004) A-Z of quantitative PCR. International University Line, La Jolla.
  2. Nolan T, Hands RE, Bustin SA (2006). Quantification of mRNA using real-time RT-PCR, Nat Protoc, 1:1559-1582
  3. Shipley, G.L. (2005) Real-Time Quantitative PCR: Theory and Practice. In Encyclopedia of molecular cell biology and molecular medicine (R. A. Meyers, Ed.), Vol. 11, pp. 481-523. Wiley-VCH Verlag, Weinheim.
  4. Shipley, G.L. (2006) Chapter 1: An Introduction to Real-Time PCR. In Real-Time PCR (Dorak MT (Ed), New York, Taylor & Francis Group.
  5. Stephen A Bustin, Vladimir Benes, Jeremy A Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory L Shipley, Jo Vandesompele, and Carl T Wittwer (2009).The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR, Clinical Chemistry, 55, 609-620.
  6. Vadesompele, J, De Preter, K, Pattyn, F, Van Roy, N, De Paepe, A, Speleman, F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol, 3(7):RESEARCH0034. Epub 2002, Jun 18.
  7. Mestdagh, P, Van Vlierberghe, P, De Weer, A, Muth, D, Westermann, F, Speleman, F and Vandesompele, J. A novel and universal method for microRNA RT-qPCR data normalization. Genome Biol. 10(6):R64. Epub 2009 Jun 16.

Proteins

  1. Voller, A, A. Bartlett and D.E. Bidwell (1978). Enzyme immunoassays with special reference to ELISA techniques, J. Clinical Pathology, 31,507-520.
  2. Clark, M.F. and A.N. Adams (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses, J. Gen. Virol, 34, 475-483.
  3. Gowan, S.M., et al. (2007) Application of Meso Scale Technology for the Measurement of Phosphoproteins in Human Tumor Xenografts. Assay and Drug Dev Technol. 5 (3):391-401.
  4. Chen, J.H., Zhang, Z.M. Stebbins J.L., Zhang, X.Y., Hoffman, R., Moore, A., Pellecchia, M. (2007) A Fragment-Based Approach for the Discovery of Isoform-Specific p38 Inhibitors. ACS Chem Bio. (2(5):329-336.
  5. Garber, E.A., O'Brien, T.W. (2008) Detection of ricin in food using electrochemiluminescence-based technology. J AOAC Int. 91(2):376-82.

Upcoming Meetings:

Nucleic Acids -
clearAdvances in qPCR
clearSelect Biosciences
clearBoston, MA
clearApril 2012
clear http://www.selectbiosciences.com/conferences/AqPCR2012/

Proteins -
clearUS HUPO
clearFrom genes to function
clearMarch 4-7, 2012
clearSan Francisco, CA
clearhttp://www.ushupo.org/Home/tabid/36/Default.aspx

Useful Websites:

The following web site updated regularly by Michael Pfaffl in Freising-Weinstephan, Germany has many useful links for all things related to real-time qPCR http://www.gene-quantification.info/

A guide for assay design:

An Introduction to Designing Probe-Based Real-Time qPCR Assays — Gregory L. Shipley, Ph.D.



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