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NMR Laboratory
Structural Biology
Imaging Center

6431 Fannin, MSB-G.602

John A. Putkey
Professor
Biochemistry & Molecular Biology

713-500-6061 Office
713-500-6062 Wet Lab
713-500-5073 NMR Lab
John.Putkey@uth.tmc.edu


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NMR

Advance 600 Ultrashield NMR spectrometer

 

SYSTEM:

Nuclear Magnetic Resonance (NMR) is a highly versatile tool that allows biochemists and structural biologists to probe the nano-environment of atoms in small molecules, as well as macromolecular proteins and nucleic acids.  A key feature of NMR is that samples are studied in aqueous solutions at physiological pH and salt conditions, and it is the only technique available that can provide atomic resolution structures of proteins and nucleic acids in their native solution states.  NMR can also probe structural dynamics and conformational transitions in macromolecules on time scales ranging from picoseconds to minutes, making it particularly useful for the study of intrinsically disordered proteins, as well as pathways of protein folding and the effect of solution conditions on protein structure.  NMR is a tool of choice to map sites of interaction between macromolecules, or between proteins and small ligands.  Indeed, NMR is a primary tool used to identify lead compounds that may eventually develop into therapeutic drugs.

The UT-Houston NMR laboratory includes an AvanceTM 600 MHz UltrashieldTM NMR spectrometer with a Cryoprobe that can collect data on dilute liquid samples and under high salt conditions.  Researchers at UT-Houston are encouraged to contact the NMR Manager to discuss projects that can benefit from NMR experiments.

The AvanceTM 600 NMR spectrometer has a 54 mm bore UltrashieldTM magnet with electronics capable of monitoring 4 channels.  Available probes include the following:

  1. Cryoprobe TXI (Inverse Triple Resonance) probe (5 mm sample diameter) for observation of 1H while decoupling 13C and 15N, including 2D lock with actively shielded Z-gradient coil. Temperature range +10 to +60 °C.  Signal to noise is 1H > 6000:1 (0.1% ethyl benzene)
  2. TXI (Inverse Triple Resonance) probe (5 mm sample diameter) for observation of 1H while decoupling 13C and 15N, including 2D lock with actively shielded X, Y, and Z-gradent coils.  Temperature range -100 to +70 °C. Signal to noise is 1H > 1000:1 (0.1% ethyl benzene).
  3. Inverse broadband (BBI) probe (5 mm sample diameter), 1H observe with broadband decoupling coil tunable from 109Ag to 31P.  Temperature range is -150 to + 180 °C.  Signal to noise is 1H > 1100:1 (0.1% ethyl benzene).
  4. Inverse detect 1H triple resonance gradient probe (5 mm sample diameter) with 2D lock. The X-channel of the probe is double tuned to 13C and 31P. Temperature range is -60 to +100 °C. Signal to noise is 1H > 1100:1 (0.1% ethyl benzene)