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2	Who is Luminex? Luminex develops and manufactures xMAP^® Technology – Multiplex liquid bead arrays
3	Peer-reviewed publications 1,915 total peer-reviewed papers using Luminex technology 562 on pharmaceutical/therapeutic development 55 papers in this area in 2008, to date 21% of a total of 268 papers so far in 2008
4	2008 Selected publications Fraccarollo, D., P. Galuppo, et al. (2008). "Immediate Mineralocorticoid Receptor Blockade Improves Myocardial Infarct Healing by Modulation of the Inflammatory Response." Hypertension: HYPERTENSIONAHA.107.100941. Hegde, V. L., S. Hegde, et al. (2008). "Attenuation of experimental autoimmune hepatitis by exogenous and endogenous cannabinoids: Involvement of regulatory T cells." Mol. Pharmacol.: mol.108.047035. Huber, L. C., P. Kunzler, et al. (2008). "Effects of a novel tyrosine kinase inhibitor in rheumatoid arthritis synovial fibroblasts." Ann Rheum Dis 67(3): 389-394. Jiz, M., H.-W. Wu, et al. (2008). "Pilot Scale Production and Characterization of Paramyosin, a Vaccine Candidate for Schistosomiasis japonica." Infect. Immun.: IAI.00409-08. Mogensen, T. H., R. S. Berg, et al. (2008). "Mechanisms of Dexamethasone-Mediated Inhibition of Toll-Like Receptor Signaling Induced by Neisseria meningitidis and Streptococcus pneumoniae." Infect. Immun. 76(1): 189-197. Oh, Y., R. S. Herbst, et al. (2008). "Enzastaurin, an Oral Serine/Threonine Kinase Inhibitor, As Second- or Third-Line Therapy of Non-Small-Cell Lung Cancer." J. Clin. Oncol. 26(7): 1135-1141. Rezzoug, F., Y. Huang, et al. (2008). "TNF-{alpha} Is Critical to Facilitate Hemopoietic Stem Cell Engraftment and Function." J. Immunol. 180(1): 49-57. Richardson, M. A., A. Gupta, et al. (2008). "Treatment of Sepsis-Induced Acquired Protein C Deficiency Reverses ACE-2 Inhibition and Decreases Pulmonary Inflammatory Response." J. Pharmacol. Exp. Ther.: jpet.107.130609. Wardenburg, J. B. and O. Schneewind (2008). "Vaccine protection against Staphylococcus aureus pneumonia." J. Exp. Med.: jem.20072208. Zhong, H., B. Han, et al. (2007). "Low-Dose Paclitaxel Prior to Intratumoral Dendritic Cell Vaccine Modulates Intratumoral Cytokine Network and Lung Cancer Growth." Clin. Cancer Res. 13(18): 5455-5462.
5	Unpublished research (AACR 2008) Demonstration of Gardasil specificity by multiplex bead-based assay Merck Multiple virus-like particles conjugated to beads Specificity of Gardasil in serum tested against multiple VLPs Cell-based and cytokine-directed chemical screen predicts the drug potency with anti-multiple myeloma activities U of Pittsburgh Cytokine profile associated with malignant transformation known Drug library screened for potency in reducing expression of this profile Rational drug discovery method
6	Technology Background Technology platform basics Industry advances in multiplex and parallel analysis
7	How Does xMAP^® Technology Work? 5.6 micron microspheres
8	How Does xMAP^® Technology Work? 5.6 micron microspheres Proprietary dyes and dyeing process
9	How Does xMAP^® Technology Work? 5.6 micron microspheres Proprietary dyes and dyeing process Spectrally distinct microsphere sets
10	How Does xMAP^® Technology Work? 5.6 micron microspheres Proprietary dyes and dyeing process Spectrally distinct microsphere sets Assay chemistry on bead surface
11	How Does xMAP^® Technology Work? Assays are read using a compact microsphere analyzer
12	How Does xMAP^® Technology Work? Assays are read using a compact microsphere analyzer Analyzer samples well
13	How Does xMAP^® Technology Work? Assays are read using a compact microsphere analyzer Analyzer samples well Lasers excite fluorescent dyes Red laser for bead classification Green laser for assay result
14	How Does xMAP^® Technology Work? Assays are read using a compact microsphere analyzer Analyzer samples well Lasers excite fluorescent dyes Red laser for bead classification Green laser for assay result Multiple readings for each microsphere set
15	Applications
16	Summary Versatility A single xMAP technology-based system can perform bioassays in several different formats Nucleic acids Antigen-antibody binding Enzyme assays Receptor-ligand Open architecture system Available kits and systems “Home brew” assays Accuracy and reproducibility High-volume production of xMAP microspheres within a single lot allows assay standardization that solid-phased flat arrays cannot provide Speed/throughput Each microsphere is an individual test: a large number of different bioassays can be performed and analyzed simultaneously Focused, flexible multiplexing
17	Thank you More information at www.luminexcorp.com