Mikhail Kolonin, Ph.D.
Assistant Professor;
Centre for Stem Cell Research
phone 713.500.3146;
fax 713.500.2424
Mikhail.G.Kolonin@uth.tmc.edu
Dr. Kolonin's research is aimed at understanding how adult mesenchymal stem cells from white adipose tissue effect cancer progression in obesity.
Dr. Mikhail G. Kolonin, received his M.S. degree from Novosibirsk State University (Russia), focusing on mammalian genome mapping using somatic cell hybrids. He received his Ph.D. at Wayne State University (Detroit, MI) from the laboratory of Dr. Russell Finley Jr., which investigated the cell cycle biology through novel applications of the yeast two-hybrid system. Dr. Kolonin has pioneered expression of peptide aptamers programmed to disrupt specific protein contacts in living animals, using the Drosophila model (PNAS 1998). As a post-doctoral fellow, and later a junior faculty, in the laboratory headed by Renata Pasqualini and Wadih Arap at M. D. Anderson Cancer Center (Houston, TX), Dr. Kolonin has provided a major contribution to the project outlining the map of human vasculature with phage-displayed combinatorial peptide libraries (Nature Medicine 2002). His goal to explore receptors expressed within vascular and tumor cell surface proteomes as targets for therapy by combining proteomics and bioinformatics approaches has resulted in a number of recent high-impact publications. Dr. Kolonin has also led the development of a novel (currently licensed) obesity treatment through targeted ablation of adipose tissue (Nature Medicine 2004).
White fat tissue has been recently realized as an abundant source of adult mesenchymal stem cells that may serve as progenitor cells for tumor vasculature and thus promote cancer progression. Dr. Kolonin’s bimodal expertise in cancer and obesity cell biology merges the fields for the investigation of adipose stem cell role in cancer progression. As an integral part of the newly built Stem Cell Research Center chaired by Dr. Paul J. Simmons, he is focusing on exploring the role of adult stem cells in tumor vasculogenesis. In parallel, Dr. Kolonin is characterizing the molecular interactions that activate adipose stem cell mobilization from their niche in the fat tissue and mediate their homing to and engraftment into tumors.
SELECTED PUBLICATIONS:
Ji Y., Yuin G., Tsui K., Kolonin M. G., Sun J., Arap W., Pasqualini R., and Do K. Bayesian mixture models for complex high-dimensional count data in phage display experiments. Journal of Royal Statistical Society. In press.
Kolonin M. G., Sergeeva A., Molldrem J., Pasqualini R., and Arap W. (2006) Display technologies: application for the discovery of drug and gene delivery agents Advanced Drug Delivery Reviews, 58, 1622-1654.
Kolonin M. G. et al. (2006) Ligand-directed surface profiling of human cancer cells with combinatorial peptide libraries. Cancer Research. 66, 34-40.
Kolonin M. G. et al. (2006) Synchronous selection of homing peptides for multiple tissues by in vivo phage display. FASEB Journal, 20(7), 979-981.
Kolonin M. G., Saha P. K., Chan L., Pasqualini R., Arap W. (2004). Reversal of obesity by targeted ablation of adipose tissue. Nature Medicine, 10, 625-32.
Kolonin, M. G., Pasqualini R., and Arap W. (2003). Mapping human vascular heterogeneity by in vivo phage display. In Genetics of Angiogenesis, J.B. Hoying eds., BIOS Scientific Publishers Ltd, Oxford, UK 167-193.
Kolonin, M. G., Arap W. and Pasqualini R. (2002). Teratogenicity induced by targeting a placental immunoglobulin transporter. Proceedings of the National Academy of Sciences, USA, 99 (20):13055-60.
Arap W., Kolonin, M. G., et al. (2002). Steps toward mapping the human vasculature by phage display. Nature Medicine, 8, 121-7.
Kolonin, M. G., Pasqualini, R., and Arap, W. (2001). Molecular addresses in blood vessels as targets for therapy. Current Opinions in Chemical Biology. 5, 308-313.
Kolonin, M. G., Zhong, J., and Finley, R. L., Jr. (2000). Interaction matting methods in two-hybrid systems. Methods in Enzymology, 328, 26-46.
Kolonin, M. G. (2000). Peptide aptamers as tools to study protein function in Drosophila. Ph.D. Dissertation, Center for Molecular Medicine and Genetics, Wayne State University, 1-146.
Kolonin, M. G., and Finley, R. L., Jr. (2000). A Role for Cyclin J in the Rapid Nuclear Division Cycles of Early Drosophila Embryogenesis. Developmental Biology, 227, 661-672.
Golemis, E. A., Serebriiskii, I., Finley, R. L., Jr., Kolonin, M. G., Gyuris, J., and Brent, R. (1999). Analysis of protein interactions. In Current Protocols in Molecular Biology, F. M. Ausubel, R. Brent, R. E. Kingston, D. Morre, J. G. Seidman and K. Struhl, eds. (New York: John Wiley & Sons, Inc.).
Kolonin, M. G., and Finley, R. L., Jr. (1998). Targeting cyclin-dependent kinases in Drosophila with peptide aptamers. Proceedings of the National Academy of Sciences, USA 95, 14266-71.
Koroleva, I. V., Malchenko, S. N., Brusgaard, K., Matyakhina, L. D., Kolonin, M. G., Pack, S. D., Searle, J. B., Borodin, P. M., Serov, O. L., and Bendixen, C. (1996). Chromosome localization of the gene for ornithine transcardomylase in the common shrew (Sorex araneus). Hereditas, 125, 239-241.
Malchenko, S. N., Koroleva, I. V., Brusgaard, K., Matyakhina, L. D., Kolonin, M. G., Pack, S. D., Searle, J. B., Borodin, P. M., Serov, O. L., and Bendixen, C. (1996). Chromosome localization of the gene for growth hormone in the common shrew (Sorex araneus). Hereditas, 125, 243-5.
Matyakhina, L. D., Kolonin, M. G., Pack, S. D., Borodin, P. M., Searle, J. B., and Serov, O. L. (1996). Chromosome localization of the loci for PEPA, PEPB, PEPS, IDH1, GSR, MPI, PGM1, NP, SOD1, and ME1 in the common shrew (Sorex araneus). Mammalian Genome, 7, 265-7.
Pack, S. D., Kolonin, M. G., Borodin, P. M., Searle, J. B., and Serov, O. L. (1995). Gene mapping in the common shrew (Sorex araneus; Insectivora) by shrew-rodent cell hybrids: chromosome localization of the loci for HPRT, TK, LDHA, MDH1, G6PD, PGD, and ADA. Mammalian Genome, 6, 784-7.