Development of BK channels in neocortical pyramidal neurons.
Kang, Jian John R. Huguenard and David A. Prince.
Department of Neurology and Neurological Sciences, Stanford University,
Stanford, CA 94305.
APStracts 3:0026N, 1996.
SUMMARY AND CONCLUSIONS
1. Postnatal development of a large conductance Ca2+-activated K+ channel (BK
channel) was investigated in neocortical infragranular pyramidal neurons with
inside-out and outside-out patch clamp configurations. Neurons were acutely
isolated from slices of 1-28 day old rats (P1- P28) by using a vibrating glass
probe after preincubation with low concentrations of enzymes. Patch membrane
area was estimated by measuring membrane capacitance. The density,
distribution, voltage-dependence, Ca2+-sensitivity, kinetics and
pharmacological properties of BK channels were examined in neurons from
animals of different ages. 2. In somata, the density of BK channels was
0.056+/-0.011/mm2 in P1 neurons and 0.312+/-0.008/mm2 in P28 neurons. There
was an abrupt increase between P5 and P7 at a rate of 0.042/mm2/day. Before P5
and after P7, the density of BK channels also increased but at slower rates.
3. The density of BK channels in proximal apical dendrites underwent a similar
developmental sequence. There was a relatively large increase between P5 and
P7 with a rate of 0.021 /mm2/day, and after P7, channel density increased more
slowly (0.002/mm2/day). In P1 neurons, channel density in apical dendrites was
0.039+/-0.008/mm2, which was close to that in somata, while in P28 neurons,
channel density (0.134+/-0.008/mm2) was less than half of that in somata. 4.
The distribution of BK channels was different in immature and mature neurons.
In somata of P1 neurons, BK channels were distributed singly without evidence
of clustering, whereas in P28 neurons BK channels were clustered in groups of
4. BK channels in both P1 and P14 neurons showed a steep increase in the
probability of opening (Po) as intracellular Ca2+ concentration was raised
from 50 nM to 100 nM, especially at positive membrane potentials. The Ca2+-
dependence, as measured by the [Ca2+]i that provided half maximal Po at a
variety of membrane potentials, was not different in patches from P1 and P14
neurons. On the other hand, the voltage-dependence of BK channels shifted
during ontogeny such that Po was larger at negative potentials in P14 than in
P1 neurons. 6. The voltage dependence of P1 BK channels was bimodally
distributed with 57% of channels exhibiting an immature pattern consisting of
a more positive V1/2 and a smaller change in voltage required to produce an e-
fold increase in Po. Immature type P1 BK channels showed a longer mean closed
time at negative membrane potentials than either P14 or mature P1 BK channels.
7. No postnatal developmental changes in pharmacological properties of BK
channels were observed. In both mature and immature neurons, BK channels were
partially inhibited by 30 nM or 100 nM charybdotoxin (ChTX) and fully blocked
by 1 mM ChTX. The IC50 for ChTX was 100 nM, indicating that BK channels in
neocortical pyramidal neurons are much less sensitive to ChTX than those in
muscle cells and sympathetic ganglion neurons. BK channels were also inhibited
by 0.5 mM TEA and 50 mM trifluoperazine. 8. These data indicate that
functional somatic and dendritic BK channels are inserted into neuronal
membranes during neocortical development, with an especially rapid increment
in density occurring around P5 - P7. These changes, which occur at a time when
other voltage-gated ion channels are known to be increasing in density,
contribute to the development of neocortical excitability.
Received 27 October 1995; accepted in final form 3 January 1996.
APS Manuscript Number J724-5.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 29 January 96