ADULT THALAMOCORTICAL TRANSMISSION INVOLVES BOTH NMDA AND non-NMDA
Gil, Ziv and Yael Amitai.
Dept. of Physiology, Zlotowski Center for Neuroscience, Faculty of Health
Sciences, Ben-Gurion University, Beer-Sheva, ISRAEL.
APStracts 3:0091N, 1996.
SUMMARY AND CONCLUSIONS
1. The involvement of NMDA receptors in thalamocortical transmission has been
demonstrated in early postnatal development, but could not be determined so
far in adult animals. We used thalamocortical slices from brains of mature
mice to examine whether NMDA receptors exist in adult thalamocortical
synapses, and what is their potential contribution to thalamocortical synaptic
responses. 2. Thalamocortical fibers to the primary somatosensory area of the
cortex were stimulated by an electrode placed in the ventro-basal (VB) nucleus
of the thalamus. Horizontal intracortical axons within layer 5 were stimulated
for comparison: A cut was made in the slice between layer 4 and 5 lateral to
the recording area, and a second stimulating electrode was placed under the
cut to activate these fibers. 3. Mg 2+ -free perfusing solution was used to
enhance NMDA receptor-mediated EPSPs. It led to synchronized population events
that could be evoked by stimulating either in the thalamus or in layer 6. A
short-latency, monosynaptic EPSP preceded the synchronous events in layer 5
cells. 4. Bath application of the non-NMDA antagonist 6-cyano-7-
nitroquinoxaline-2,3-dione (CNQX, 10 [mu]M) abolished only synchronized events
triggered from the thalamus, but not intracortically. CNQX reduced, but did
not abolish, the monosynaptic thalamocortical EPSP, and had almost no effect
on intracortically evoked EPSPs. Focal application of the GABA A receptor
antagonist bicuculline methiodide (BMI, 10 [mu]M) to layer 4 re-established
the appearance of synchronous events upon thalamic stimulation. 5.
Monosynaptic thalamocortical and intracortical EPSPs on the same cells were
studied in the presence of normal [Mg 2+ ] to mimic better the physiological
state. Spikes were abolished by intracellular injection of the sodium channel
blocker QX-314. EPSPs from both sources were reduced compared to control, but
not blocked, after bath application of CNQX (10 [mu]M). CNQX-resistant EPSPs
of both synaptic tracts were evident at membrane potentials close to rest,
exhibited strong voltage-dependency and were blocked by the NMDA antagonist
DL-2-amino-5-phosphonovaleric acid (APV), suggesting they were NMDA receptor-
dependent. 6. To confirm that NMDA receptors were indeed activated at
thalamocortical synapses and not by antidromic activation of intracortical
pathways, we used pressure microapplications of glutamate (10 mM) to nucleus
VB of the thalamus. The perfusing solution contained CNQX (10 [mu]M) and cells
were recorded with micropipettes containing QX-314. All cells that showed a
monosynaptic response to electrical thalamic stimulation, also exhibited a
barrage of mixed synaptic responses to thalamic glutamate application. The
amplitude of these synaptic events was dependent strongly on the membrane
voltage, and the application of APV to the cortex abolished them completely.
7. Our results demonstrate that, in adult animals, both thalamocortical and
intracortical synaptic pathways utilize NMDA as well as non-NMDA receptors.
Received 17 January 1996; accepted in final form 30 April 1996.
APS Manuscript Number J26-6.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 28 May 96